Recombinant Human PAPPA protein, His-tagged

• Cat.No.: PAPPA-7722H
• Product Overview: Recombinant Human PAPPA aa. (Thr1270~Cys1558 (Accession # Q13219)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Thr1270~Cys1558 (Accession # Q13219)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PAPPA pappalysin 1 [ Homo sapiens (human) ]
• Official Symbol: PAPPA
• Synonyms: PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase; IGF-dependent IGFBP-4 protease; aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; insulin-like growth factor-dependent IGF binding protein-4 protease; pregnacy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1; pappalysin-1
• Gene ID: 5069
• mRNA Refseq: NM_002581.4
• Protein Refseq: NP_002572.2
• UniProt ID: Q13219