Recombinant Human parainfluenza 3 virus (HPIV-3) (strain Wash/47885/57) HN protein(Asn54-Ser572), His-tagged

• Cat.No.: HN-3262V
• Product Overview: Recombinant Human paraInfluenza 3 virus (HPIV-3) (strain Wash/47885/57) HN (P08492-1) (Asn54-Ser572) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: HPIV-3
• Tag: N-His
• Protein length: Asn54-Ser572
• Form: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Human parainfluenza 3 virus (HPIV-3) (strain Wash/47885/57) HN consists of 537 amino acids and predicts a molecular mass of 60.4 kDa. It migrates as an approximately 61.9 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.