Recombinant Human PCSK2 Protein, His-tagged

• Cat.No.: PCSK2-2563H
• Product Overview: Recombinant Human PCSK2 Protein (Thr461-Ala597) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.9 kDa
  Accurate Molecular Mass: 18 kDa
• Protein length: Thr461-Ala597
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PCSK2 proprotein convertase subtilisin/kexin type 2 [ Homo sapiens (human) ]
• Official Symbol: PCSK2
• Synonyms: PCSK2; proprotein convertase subtilisin/kexin type 2; NEC2; neuroendocrine convertase 2; KEX2 like endoprotease 2; PC2; SPC2; prohormone convertase 2; KEX2-like endoprotease 2; NEC 2; NEC-2
• Gene ID: 5126
• mRNA Refseq: NM_001201528
• Protein Refseq: NP_001188457
• MIM: 162151
• UniProt ID: P16519