Recombinant Human PDCD1LG2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: PDCD1LG2-6844H
• Product Overview: Biotinylated Recombinant Human PDCD1LG2 protein(Q9BQ51-1)(Leu20-Thr220), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu20-Thr220
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: PDCD1LG2 programmed cell death 1 ligand 2 [ Homo sapiens ]
• Official Symbol: PDCD1LG2
• Synonyms: PDCD1LG2; programmed cell death 1 ligand 2; B7 dendritic cell molecule; B7 DC; bA574F11.2; Btdc; CD273; PD L2; PDL2; B7-DC; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2; B7DC; PD-L2; PDCD1L2; MGC142238; MGC142240
• Gene ID: 80380
• mRNA Refseq: NM_025239
• Protein Refseq: NP_079515
• MIM: 605723
• UniProt ID: Q9BQ51