Recombinant Human PDHA1 protein, His&GST-tagged

• Cat.No.: PDHA1-245H
• Product Overview: Recombinant Human PDHA1 protein(P08559-1) (Phe30-Ser390) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 30-390 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, 3mM DTT, 10% gly, pH 8.0.
• Molecular Mass: The recombinant human PDHA1/GST chimera consists of 598 amino acids and has a calculated molecular mass of 68 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 [ Homo sapiens ]
• Official Symbol: PDHA1
• Synonyms: PDHA1; pyruvate dehydrogenase (lipoamide) alpha 1; PDHA; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; PHE1A; PDHCE1A
• Gene ID: 5160
• mRNA Refseq: NM_000284
• Protein Refseq: NP_000275
• MIM: 300502
• UniProt ID: P08559