Active Recombinant Human PECAM1 protein, His-tagged

• Cat.No.: PECAM1-647H
• Product Overview: Active Recombinant Human PECAM1 protein(P16284)(28~601 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 28-601 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Platelet/Endothelial Cell Adhesion Molecule (PECAM1), also known as CD31, is a transmembrane glycoprotein which belongs to the immunoglobulin superfamily. PECAM1 is widely expressed in various tissues, including blood vessels, the heart, lungs, and the brain and plays an important role in intercellular adhesion, cell migration, platelet function regulation and immune response.Abnormal expression of PECAM-1 is also associated with a variety of diseases, such as atherosclerosis, inflammation and blood diseases. Besides, Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) has been identified as an interactor of PECAM1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PECAM1 and recombinant human PTPN6. Briefly, biotin-linked PECAM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PTPN6-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human PECAM1 and recombinant human PTPN6 was shown, the EC50 for this effect is 1.69 ug/mL.
• Molecular Mass: 65.6kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PECAM1 platelet/endothelial cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: PECAM1
• Synonyms: PECAM1; platelet/endothelial cell adhesion molecule 1; platelet/endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; CD31; CD31 antigen; PECA1; GPIIA; PECAM-1; endoCAM; CD31/EndoCAM; FLJ34100; FLJ58394
• Gene ID: 5175
• mRNA Refseq: NM_000442
• Protein Refseq: NP_000433
• MIM: 173445
• UniProt ID: P16284