Recombinant Human PFKM, His-GST tagged

• Cat.No.: PFKM-8640H
• Product Overview: Recombinant Human PFKM (P08237-1) (Thr2-Val780), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 2-780 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerolNormally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PFKM /GST chimera consists of 1016 amino acids and has a calculated molecular mass of 112.9 kDa. The recombinant protein migrates as an approximately 113 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PFKM phosphofructokinase, muscle [ Homo sapiens ]
• Official Symbol: PFKM
• Gene ID: 5213
• mRNA Refseq: NM_000289
• Protein Refseq: NP_000280
• MIM: 610681
• UniProt ID: P08237
• Chromosome Location: 12q13.11