Recombinant Human PFKM protein, His&GST-tagged

• Cat.No.: PFKM-2375H
• Product Overview: Recombinant Human PFKM protein(P08237-1) (Thr2-Val780) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 2-780 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol.
• Molecular Mass: The recombinant human PFKM /GST chimera consists of 1016 amino acids and has a calculated molecular mass of 112.9 kDa. The recombinant protein migrates as an approximately 113 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PFKM phosphofructokinase, muscle [ Homo sapiens ]
• Official Symbol: PFKM
• Synonyms: PFKM; phosphofructokinase, muscle; PFKX, phosphofructokinase, polypeptide X; 6-phosphofructokinase, muscle type; PFK 1; PFK-A; phosphohexokinase; phosphofructokinase 1; phosphofructokinase-M; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase, polypeptide X; GSD7; PFK1; PFKA; PFKX; PFK-1; MGC8699
• Gene ID: 5213
• mRNA Refseq: NM_000289
• Protein Refseq: NP_000280
• MIM: 610681
• UniProt ID: P08237