Recombinant Human PFKP Protein, MYC/DDK-tagged
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| Cat.No. : | PFKP-01H |
| Product Overview : | Recombinant protein of human phosphofructokinase, platelet (PFKP) with a C-terminal MYC/DDK tag was expressed in HEK293T. |
| Description : | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. |
| Source : | HEK293T |
| Species : | Human |
| Tag : | Myc/DDK |
| Bio-activity : | Cell treatment. |
| Molecular Mass : | 85.4 kDa |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : | Store at -80 centigrade. |
| Concentration : | >50 μg/mL as determined by microplate BCA method |
| Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Figure : |
PFKP promotes the proliferation of human pulmonary artery smooth muscle cells (HPASMCs). HPASMCs were serum-starved for 48 hours and stimulated with recombinant PFKP protein (100 ng/mL) for 72 hours. Fixed cells stained with crystal violet were counted to assess cell proliferation. |
| Coomassie blue staining of purified PFKP protein. : |
The protein was produced from HEK293T cells transfected with PFKP cDNA clone using MegaTran 2.0. |
| Gene Name : | PFKP phosphofructokinase, platelet [ Homo sapiens (human) ] |
| Official Symbol : | PFKP |
| Synonyms : | PFKP; phosphofructokinase, platelet; PFKF; PFK-C; PFK-P; ATP-PFK; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase; EC 2.7.1.11 |
| Gene ID : | 5214 |
| mRNA Refseq : | NM_002627 |
| Protein Refseq : | NP_002618 |
| MIM : | 171840 |
| UniProt ID : | Q01813 |
◆ Recombinant Protein
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| PFKP-3156C | Recombinant Chicken PFKP | +Inquiry |
| Pfkp-1737M | Recombinant Mouse Pfkp protein, His & T7-tagged | +Inquiry |
| PFKP-30890TH | Recombinant Human PFKP, His-tagged | +Inquiry |
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◆ Lysates
| PFKP-3270HCL | Recombinant Human PFKP 293 Cell Lysate | +Inquiry |
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