Recombinant Human PFKP Protein, MYC/DDK-tagged


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Recombinant Human PFKP Protein, MYC/DDK-tagged

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Cat.No. : PFKP-01H
Product Overview : Recombinant protein of human phosphofructokinase, platelet (PFKP) with a C-terminal MYC/DDK tag was expressed in HEK293T.
Description : This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
Source : HEK293T
Species : Human
Tag : Myc/DDK
Bio-activity : Cell treatment.
Molecular Mass : 85.4 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Figure :

PFKP promotes the proliferation of human pulmonary artery smooth muscle cells (HPASMCs). HPASMCs were serum-starved for 48 hours and stimulated with recombinant PFKP protein (100 ng/mL) for 72 hours. Fixed cells stained with crystal violet were counted to assess cell proliferation.

Coomassie blue staining of purified PFKP protein. :

The protein was produced from HEK293T cells transfected with PFKP cDNA clone using MegaTran 2.0.

Gene Name : PFKP phosphofructokinase, platelet [ Homo sapiens (human) ]
Official Symbol : PFKP
Synonyms : PFKP; phosphofructokinase, platelet; PFKF; PFK-C; PFK-P; ATP-PFK; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase; EC
Gene ID : 5214
mRNA Refseq : NM_002627
Protein Refseq : NP_002618
MIM : 171840
UniProt ID : Q01813
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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