Recombinant Human PGGT1B Protein, His-tagged

• Cat.No.: PGGT1B-2584H
• Product Overview: Recombinant Human PGGT1B Protein (Met1-Thr377) with a N-His tag was expressed in E. coli.

Specification

• Description: Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine (summarized by Zhang et al., 1994 [PubMed 8106351]). The enzyme is composed of a 48-kD alpha subunit (FNTA; MIM 134635) and a 43-kD beta subunit, encoded by the PGGT1B gene. The FNTA gene encodes the alpha subunit for both GGTase-I and the related enzyme farnesyltransferase.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 46.1 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Met1-Thr377
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PGGT1B protein geranylgeranyltransferase type I, beta subunit [ Homo sapiens (human) ]
• Official Symbol: PGGT1B
• Synonyms: PGGT1B; protein geranylgeranyltransferase type I, beta subunit; geranylgeranyl transferase type-1 subunit beta; BGGI; GGTI; GGTase-I-beta; geranylgeranyltransferase type I beta subunit; geranylgeranyl transferase type I subunit beta; type I protein geranyl-geranyltransferase subunit beta
• Gene ID: 5229
• mRNA Refseq: NM_005023
• Protein Refseq: NP_005014
• MIM: 602031
• UniProt ID: P53609