Recombinant Human PIKFYVE Protein, His-tagged

• Cat.No.: PIKFYVE-359H
• Product Overview: Recombinant Human PIKFYVE, transcript variant 4, fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[
• Source: E. coli
• Tag: His
• Form: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.
• Molecular Mass: 62 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: PIKFYVE phosphoinositide kinase, FYVE finger containing [ Homo sapiens ]
• Official Symbol: PIKFYVE
• Synonyms: PIKFYVE; phosphoinositide kinase, FYVE finger containing; phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase, type III , PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; KIAA0981; MGC40423; p235; PIKfyve; PIP5K; PIPkin-III; type III PIP kinase; phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; CFD; FAB1; PIP5K3; FLJ37746
• Gene ID: 200576
• mRNA Refseq: NM_001178000
• Protein Refseq: NP_001171471
• MIM: 609414
• UniProt ID: Q9Y2I7