Recombinant Human PIP4K2A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PIP4K2A-1563H
• Product Overview: PIP4K2A MS Standard C13 and N15-labeled recombinant protein (NP_005019) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.2 kDa
• AA Sequence: MATPGNLGSSVLASKTKTKKKHFVA QKVKLFRASDPLLSVLMWGVNHSIN ELSHVQIPVMLMPDDFKAYSKIKVD NHLFNKENMPSHFKFKEYCPMVFRN LRERFGIDDQDFQNSLTRSAPLPND SQARSGARFHTSYDKRYIIKTITSE DVAEMHNILKKYHQYIVECHGITLL PQFLGMYRLNVDGVEIYVIVTRNVF SHRLSVYRKYDLKGSTVAREASDKE KAKELPTLKDNDFINEGQKIYIDDN NKKVFLEKLKKDVEFLAQLKLMDYS LLVGIHDVERAEQEEVECEENDGEE EGESDGTHPVGTPPDSPGNTLNSSP PLAPGEFDPNIDVYGIKCHENSPRK EVYFMAIIDILTHYDAKKKAAHAAK TVKHGAGAEISTVNPEQYSKRFLDF IGHILTTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PIP4K2A phosphatidylinositol-5-phosphate 4-kinase type 2 alpha [ Homo sapiens (human) ]
• Official Symbol: PIP4K2A
• Synonyms: PIP4K2A; phosphatidylinositol-5-phosphate 4-kinase, type II, alpha; phosphatidylinositol 4 phosphate 5 kinase, type II, alpha, PIP5K2A; phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; PIP5KIIA; PIP5KIIalpha; PIP5KIII; PIP4KII-alpha; PI(5)P 4-kinase type II alpha; PtdIns(4)P-5-kinase B isoform; ptdIns(4)P-5-kinase C isoform; diphosphoinositide kinase 2-alpha; ptdIns(5)P-4-kinase isoform 2-alpha; 1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-4-phosphate-5-kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform; PIPK; PI5P4KA; PIP5K2A; FLJ13267; PIP5KII-alpha
• Gene ID: 5305
• mRNA Refseq: NM_005028
• Protein Refseq: NP_005019
• MIM: 603140
• UniProt ID: P48426