Recombinant Human PKLR Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PKLR-2785H
• Product Overview: PKLR MS Standard C13 and N15-labeled recombinant protein (NP_000289) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 61.8 kDa
• AA Sequence: MSIQENISSLQLRSWVSKSQRDLAK SILIGAPGGPAGYLRRASVAQLTQE LGTAFFQQQQLPAAMADTFLEHLCL LDIDSEPVAARSTSIIATIGPASRS VERLKEMIKAGMNIARLNFSHGSHE YHAESIANVREAVESFAGSPLSYRP VAIALDTKGPEIRTGILQGGPESEV ELVKGSQVLVTVDPAFRTRGNANTV WVDYPNIVRVVPVGGRIYIDDGLIS LVVQKIGPEGLVTQVENGGVLGSRK GVNLPGAQVDLPGLSEQDVRDLRFG VEHGVDIVFASFVRKASDVAAVRAA LGPEGHGIKIISKIENHEGVKRFDE ILEVSDGIMVARGDLGIEIPAEKVF LAQKMMIGRCNLAGKPVVCATQMLE SMITKPRPTRAETSDVANAVLDGAD CIMLSGETAKGNFPVEAVKMQHAIA REAEAAVYHRQLFEELRRAAPLSRD PTEVTAIGAVEAAFKCCAAAIIVLT TTGRSAQLLSRYRPRAAVIAVTRSA QAARQVHLCRGVFPLLYREPPEAIW ADDVDRRVQFGIESGKLRGFLRVGD LVIVVTGWRPGSGYTNIMRVLSIST RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PKLR pyruvate kinase L/R [ Homo sapiens (human) ]
• Official Symbol: PKLR
• Synonyms: PKLR; pyruvate kinase, liver and RBC; pyruvate kinase isozymes R/L; pyruvate kinase 1; pyruvate kinase type L; pyruvate kinase isozyme R/L; R-type/L-type pyruvate kinase; red cell/liver pyruvate kinase; pyruvate kinase, liver and blood cell; PK1; PKL; PKR; RPK; PKRL
• Gene ID: 5313
• mRNA Refseq: NM_000298
• Protein Refseq: NP_000289
• MIM: 609712
• UniProt ID: P30613