Recombinant Human PKN1 protein, His & T7-tagged

• Cat.No.: PKN1-2709H
• Product Overview: Recombinant Human PKN1 aa. (Phe615~Phe874 (Accession # Q16512)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Phe615~Phe874
• Description: The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.9kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PKN1 protein kinase N1 [ Homo sapiens (human) ]
• Official Symbol: PKN1
• Synonyms: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA; protease-activated kinase 1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase PKN-alpha; serine-threonine kinase N; serine/threonine protein kinase N; serine/threonine-protein kinase N1
• Gene ID: 5585
• mRNA Refseq: NM_002741.3
• Protein Refseq: NP_002732.3
• UniProt ID: Q16512