Recombinant Human PLA2G7 Protein, His-tagged PLA2G7-37H

Recombinant Human PLA2G7 Protein, His-tagged

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Recombinant Human PLA2G7 Protein, His-tagged

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Cat.No. : PLA2G7-37H
Product Overview : Recombinant human PLA2G7(42-441) protein with a N-terminal His-tag was expressed in E. coli.
Description : The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 46.4 kDa
Protein length : 42-441
Purity : ≥85% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 μmol of TNB per minute at 37 centigrade in buffer containing 100 mM Tris-HCl, pH 7.2, and 300 μM of the substrate 2-Thio PAF.
Stability : ≥ 2 years
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM sodium phosphate pH 7.2, 100 mM NaCl, 20% glycerol
SDS-PAGE Analysis of PLA2G7 :

Lane 1: MW Standards; Lane 2: PAF-AH (4 μg); Lane 3: PAF-AH (2 μg). Representative gel image shown; actual purity may vary between each batch.

PLA2G7 Activity Assay :

Gene Name : PLA2G7 phospholipase A2 group VII [ Homo sapiens (human) ]
Official Symbol : PLA2G7
Synonyms : PLA2G7; phospholipase A2 group VII; PAFAD; PAFAH; LP-PLA2; LDL-PLA2; platelet-activating factor acetylhydrolase; ; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; LDL-PLA(2); LDL-associated phospholipase A2; PAF 2-acylhydrolase; PAF acetylhydrolase; gVIIA-PLA2; group-VIIA phospholipase A2; lipoprotein-associated phospholipase A2; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Gene ID : 7941
mRNA Refseq : NM_001168357
Protein Refseq : NP_001161829
MIM : 601690
UniProt ID : Q13093

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