Recombinant Human PLD6 Protein (1-252 aa), His-SUMO-tagged
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|Product Overview :||Recombinant Human PLD6 Protein (1-252 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.|
|Description :||Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.|
|Source :||E. coli|
|Form :||Tris-based buffer,50% glycerol|
|Molecular Mass :||44.3 kDa|
|Protein length :||1-252 aa|
|AA Sequence :||MGRLSWQVAAAAAVG LALTLEALPWVLRWL RSRRRRPRREALFFP SQVTCTEALLRAPGA ELAELPEGCPCGLPH GESALSRLLRALLAA RASLDLCLFAFSSPQ LGRAVQLLHQRGVRV RVVTDCDYMALNGSQ IGLLRKAGIQVRHDQ DPGYMHHKFAIVDKR VLITGSLNWTTQAIQ NNRENVLITEDDEYV RLFLEEFERIWEQFN PTKYTFFPPKKSHGS CAPPVSRAGGRLLSW HRTCGTSSESQT|
|Purity :||> 90% as determined by SDS-PAGE.|
|Notes :||Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.|
|Storage :||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.|
|Concentration :||A hardcopy of COA with reconstitution instruction is sent along with the products.|
|UniProt ID :||Q8N2A8|
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.