Recombinant Human PMEL Protein (25-467 aa), His-tagged

• Cat.No.: PMEL-1518H
• Product Overview: Recombinant Human PMEL Protein (25-467 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Metabolism. Protein Description: Partial.

Specification

• Description: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 49.0 kDa
• Protein length: 25-467 aa
• AA Sequence: KVPRNQDWLGVSRQLRTKAWNRQLY PEWTEAQRLDCWRGGQVSLKVSNDG PTLIGANASFSIALNFPGSQKVLPD GQVIWVNNTIINGSQVWGGQPVYPQ ETDDACIFPDGGPCPSGSWSQKRSF VYVWKTWGQYWQVLGGPVSGLSIGT GRAMLGTHTMEVTVYHRRGSRSYVP LAHSSSAFTITDQVPFSVSVSQLRA LDGGNKHFLRNQPLTFALQLHDPSG YLAEADLSYTWDFGDSSGTLISRAL VVTHTYLEPGPVTAQVVLQAAIPLT SCGSSPVPGTTDGHRPTAEAPNTTA GQVPTTEVVGTTPGQAPTAEPSGTT SVQVPTTEVISTAPVQMPTAESTGM TPEKVPVSEVMGTTLAEMSTPEATG MTPAEVSIVVLSGTTAAQVTTTEWV ETTARELPIPEPEGPDASSIMSTES ITGSLGPLLDGTATLRLV
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: PMEL premelanosome protein [ Homo sapiens ]
• Official Symbol: PMEL
• Synonyms: PMEL; D12S53E; gp100; Pmel17; SI; P1; SIL; ME20; P100; SILV; ME20M; ME20-M; PMEL17
• Gene ID: 6490
• mRNA Refseq: NM_001200053
• Protein Refseq: NP_001186982
• MIM: 155550
• UniProt ID: P40967