Recombinant Human POLR2J3 Protein, Myc/DDK-tagged, C13 and N15-labeled POLR2J3-5216H

Specification Gene Information Related Products

Cat.No. :	POLR2J3-5216H
Product Overview :	POLR2J3 MS Standard C13 and N15-labeled recombinant protein (NP_001091084) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description :	This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.
Source :	HEK293
Species :	Human
Tag :	Myc/DDK
Molecular Mass :	12.9 kDa
AA Sequence :	MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTMNKEDHT LGNIIKSQLL KDPQVLFAGY KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLPTRTRP LEQKLISEED LAANDILDYK DDDDKV
Purity :	> 80% as determined by SDS-PAGE and Coomassie blue staining
Stability :	Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage :	Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration :	50 μg/mL as determined by BCA
Storage Buffer :	100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name :	POLR2J3 RNA polymerase II subunit J3 [ Homo sapiens (human) ]
Official Symbol :	POLR2J3
Synonyms :	POLR2J3; polymerase (RNA) II (DNA directed) polypeptide J3; POLR2J2; RPB11b1; RPB11b2; DNA-directed RNA polymerase II subunit RPB11-b2; RNA polymerase II subunit B11-b2; DNA-directed RNA polymerase II subunit 11; DNA-directed RNA polymerase II subunit J3
Gene ID :	548644
mRNA Refseq :	NM_001097615
Protein Refseq :	NP_001091084
UniProt ID :	Q9GZM3