Recombinant Human PPIB protein, His-tagged

• Cat.No.: PPIB-652H
• Product Overview: Recombinant Human PPIB (Asp34-Ala212) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Asp34-Ala212
• Description: Peptidyl-prolyl cis-trans isomerase B (PPIB or PPIase B) is also known as Cyclophilin B (CYPB), Rotamase B, S-cyclophilin (SCYLP), is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum, which belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIB / CYPB contains one PPIase cyclophilin-type domain. PPIases accelerate the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
• Predicted N Terminal: Asp34
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 20.7 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  Further Characterization of the Target of a Potential Aptamer Biomarker for Pancreatic Cancer: Cyclophilin B and Its Posttranslational Modifications. (2013)

Gene Information

• Gene Name: PPIB
• Official Symbol: PPIB
• Synonyms: PPIB; peptidylprolyl isomerase B (cyclophilin B); peptidyl-prolyl cis-trans isomerase B; CYPB; OI9; PPIase B; rotamase B; S-cyclophilin; cyclophilin-like protein; SCYLP; CYP-S1; MGC2224; MGC14109
• Gene ID: 5479
• mRNA Refseq: NM_000942
• Protein Refseq: NP_000933
• MIM: 123841
• UniProt ID: P23284

Citation

Further Characterization of the Target of a Potential Aptamer Biomarker for Pancreatic Cancer: Cyclophilin B and Its Posttranslational Modifications

Journal: Nucleic Acid Therapeutics    PubMed ID: 24152208    Data: 2013/12/1

Authors: Partha Ray, Bruce A. Sullenger, Rebekah R. White

Article Snippet:The C-terminal endoplasmic reticulum (ER) retention motif (Ile 213–Glu 216) was deleted, and the secreted protein was purified from the conditioned media.The C-terminal endoplasmic reticulum (ER) retention motif (Ile 213–Glu 216) was deleted, and the secreted protein was purified from the conditioned media.. The proteins (samples 1 and 2) were purchased from Creative BioMart (PPIB-652H) and Sino Biological Inc. (11004-H08H) (sample 3).. Human CypB (Leu 26–Glu 216) expressed in the bacterial Escherichia coli system (sample 5) was purchased from Prospect (ENZ-313).Human CypB (Leu 26–Glu 216) expressed in the bacterial Escherichia coli system (sample 5) was purchased from Prospect (ENZ-313).