Recombinant Human PPIB protein, His-tagged

• Cat.No.: PPIB-652H
• Product Overview: Recombinant Human PPIB protein(NP_000933.1) (Asp 34-Ala 212) was expressed in HEK293, fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 34-212 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human PPIB consists of 190 amino acids and migarates as an apximately 22 kDa band in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000933.1
• Unit ID: P23284
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P23284
• Publications:
  • Further Characterization of the Target of a Potential Aptamer Biomarker for Pancreatic Cancer: Cyclophilin B and Its Posttranslational Modifications. (2013)

Gene Information

• Gene Name: PPIB peptidylprolyl isomerase B (cyclophilin B) [ Homo sapiens ]
• Official Symbol: PPIB
• Synonyms: PPIB; peptidylprolyl isomerase B (cyclophilin B); peptidyl-prolyl cis-trans isomerase B; CYPB; OI9; PPIase B; rotamase B; S-cyclophilin; cyclophilin-like protein; SCYLP; CYP-S1; MGC2224; MGC14109
• Gene ID: 5479
• mRNA Refseq: NM_000942
• protein Refseq: NP_000933
• MIM: 123841

Citation

Further Characterization of the Target of a Potential Aptamer Biomarker for Pancreatic Cancer: Cyclophilin B and Its Posttranslational Modifications

Journal: Nucleic Acid Therapeutics    PubMed ID: 24152208    Data: 2013/12/1

Authors: Partha Ray, Bruce A. Sullenger, Rebekah R. White

Article Snippet:The C-terminal endoplasmic reticulum (ER) retention motif (Ile 213–Glu 216) was deleted, and the secreted protein was purified from the conditioned media.The C-terminal endoplasmic reticulum (ER) retention motif (Ile 213–Glu 216) was deleted, and the secreted protein was purified from the conditioned media.. The proteins (samples 1 and 2) were purchased from Creative BioMart (PPIB-652H) and Sino Biological Inc. (11004-H08H) (sample 3).. Human CypB (Leu 26–Glu 216) expressed in the bacterial Escherichia coli system (sample 5) was purchased from Prospect (ENZ-313).Human CypB (Leu 26–Glu 216) expressed in the bacterial Escherichia coli system (sample 5) was purchased from Prospect (ENZ-313).