| Cat. No. : |
PPIL2-2030H |
| Description : |
PPIL2, which belongs to the cyclophilin family of peptidylprolyl isomerases, is an enzyme that plays an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. |
| Source : |
E.coli. |
| Form : |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl. |
| Purity : |
> 95% by SDS - PAGE. |
| Concentration : |
0.5 mg/ml (determined by Bradford assay). |
| Sequences Of Amino Acids : |
MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTN FRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWLKKYGTNPSNG EKLD GRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQDPTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDP SYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTG KVSASFTSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLE LHC DLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPT GTGTGGE SYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIF GRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDATTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGSSRLPDDH. |
| Biological activity : |
Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. |
| Activity Assay : |
1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant PPIL2 protein with 1ug in assay buffer.3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C. |
| Storage : |
can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Pathways : |
Ubiquitin mediated proteolysis; Hemostasis; Signaling in Immune system |
