Recombinant Human PPP1CB Protein (2-327 aa), His-tagged PPP1CB-2170H

Recombinant Human PPP1CB Protein (2-327 aa), His-tagged

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Recombinant Human PPP1CB Protein (2-327 aa), His-tagged

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Cat.No. : PPP1CB-2170H
Product Overview : Recombinant Human PPP1CB Protein (2-327 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length of Mature Protein.
Description : Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Source : E. coli
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 41.1 kDa
Protein length : 2-327 aa
AA Sequence : ADGELNVDSLITRLL EVRGCRPGKIVQMTE AEVRGLCIKSREIFL SQPILLELEAPLKIC GDIHGQYTDLLRLFE YGGFPPEANYLFLGD YVDRGKQSLETICLL LAYKIKYPENFFLLR GNHECASINRIYGFY DECKRRFNIKLWKTF TDCFNCLPIAAIVDE KIFCCHGGLSPDLQS MEQIRRIMRPTDVPD TGLLCDLLWSDPDKD VQGWGENDRGVSFTF GADVVSKFLNRHDLD LICRAHQVVEDGYEF FAKRQLVTLFSAPNY CGEFDNAGGMMSVDE TLMCSFQILKPSEKK AKYQYGGLNSGRPVT PPRTANPPKKR
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : PPP1CB protein phosphatase 1, catalytic subunit, beta isozyme [ Homo sapiens ]
Official Symbol : PPP1CB
Synonyms : PPP1CB; PP 1B; PP1beta; PP-1B; PPP1CD; MGC3672;
Gene ID : 5500
mRNA Refseq : NM_002709
Protein Refseq : NP_002700
MIM : 600590
UniProt ID : P62140

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