| Description : |
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
117 kDa |
| AA Sequence : |
MKMADAKQKRNEQLKRWIGSETDLE PPVVKRQKTKVKFDDGAVFLAACSS GDTDEVLKLLHRGADINYANVDGLT ALHQACIDDNVDMVKFLVENGANIN QPDNEGWIPLHAAASCGYLDIAEFL IGQGAHVGAVNSEGDTPLDIAEEEA MEELLQNEVNRQGVDIEAARKEEER IMLRDARQWLNSGHINDVRHAKSGG TALHVAAAKGYTEVLKLLIQAGYDV NIKDYDGWTPLHAAAHWGKEEACRI LVDNLCDMEMVNKVGQTAFDVADED ILGYLEELQKKQNLLHSEKRDKKSP LIESTANMDNNQSQKTFKNKETLII EPEKNASRIESLEQEKVDEEEEGKK DESSCSSEEDEEDDSESEAETDKTK PLASVTNANTSSTQAAPVAVTTPTV SSGQATPTSPIKKFPTTATKISPKE EERKDESPATWRLGLRKTGSYGALA EITASKEGQKEKDTAGVTRSASSPR LSSSLDNKEKEKDSKGTRLAYVAPT IPRRLASTSDIEEKENRDSSSLRTS SSYTRRKWEDDLKKNSSVNEGSTYH KSCSFGRRQDDLISSSVPSTTSTPT VTSAAGLQKSLLSSTSTTTKITTGS SSAGTQSSTSNRLWAEDSTEKEKDS VPTAVTIPVAPTVVNAAASTTTLTT TTAGTVSSTTEVRERRRSYLTPVRD EESESQRKARSRQARQSRRSTQGVT LTDLQEAEKTIGRSRSTRTREQENE EKEKEEKEKQDKEKQEEKKESETSR EDEYKQKYSRTYDETYQRYRPVSTS SSTTPSSSLSTMSSSLYASSQLNRP NSLVGITSAYSRGITKENEREGEKR EEEKEGEDKSQPKSIRERRRPREKR RSTGVSFWTQDSDENEQEQQSDTEE GSNKKETQTDSISRYETSSTSAGDR YDSLLGRSGSYSYLEERKPYSSRLE KDDSTDFKKLYEQILAENEKLKAQL HDTNMELTDLKLQLEKATQRQERFA DRSLLEMEKRERRALERRISEMEEE LKMLPDLKADNQRLKDENGALIRVI SKLSKKKKKKLDSRLRPRSTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
