"PRKAG1/PRKAB2/PRKAA1" Related Products

Recombinant Human PRKAG1 & PRKAB2 & PRKAA1 Protein, His-GST-tagged

Cat.No.: PRKAG1 & PRKAB2 & PRKAA1-1238H
Product Overview: Recombinant human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; Recombinant human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tag
Source: Baculovirus-Insect Cells
Species: Human
Tag: His-GST
Predicted N Terminal: Met & Met & His
Form: Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4.
Bio-activity: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Molecular Mass: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >94 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: PRKAG1 protein kinase, AMP-activated, gamma 1 non-catalytic subunit [ Homo sapiens ]
Official Symbol: PRKAG1
Synonyms: PRKAG1; protein kinase, AMP-activated, gamma 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK gamma-1 chain; 5-AMP-activated protein kinase, gamma-1 subunit; AMPKG; MGC8666;
Gene ID: 5571
mRNA Refseq: NM_001206709
Protein Refseq: NP_001193638
MIM: 602742
UniProt ID: P54619
Chromosome Location: 12q12-q14
Pathway: AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AM
Function: ADP binding; AMP binding; contributes_to AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; nucleotide binding; protein bindi

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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