Recombinant Human PRKCE, His-tagged

• Cat.No.: PRKCE-28941TH
• Product Overview: Recombinant Full Length Human glycosylated PKC epsilon with an amino terminal poly His-tag, produced in Sf9 insect cells. Molecular mass 87 kDa.

Specification

• Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
• Conjugation: HIS
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.03% Brij 35, 0.1M Sodium chloride, 20mM Tris, 1mM Benzamidine, 1mM DTT, 0.2mM PMSF, 0.1mM EDTA, pH 7.4
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 C2 domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.

Gene Information

• Gene Name: PRKCE protein kinase C, epsilon [ Homo sapiens ]
• Official Symbol: PRKCE
• Synonyms: PRKCE; protein kinase C, epsilon; protein kinase C epsilon type
• Gene ID: 5581
• mRNA Refseq: NM_005400
• Protein Refseq: NP_005391
• MIM: 176975
• Uniprot ID: Q02156
• Chromosome Location: 2p21
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem
• Function: ATP binding; SH3 domain binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding