Recombinant Human Pro-opiomelanocortin, His-tagged

• Cat.No.: POMC-3548H
• Product Overview: Recombinant human POMC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Pro-opiomelanocortin preproprotein, also known as POMC, a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. It regulates the corticosteroid production in the adrenal cortex. Also, POMC is cleaved into ten hormone chains named NPP, μ-MSH, ACTH, μ-MSH, CLIP, Lipotropin μ, Lipotropin μ, μ-MSH, μ endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency.
• Purity: > 85% by SDS - PAGE
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl.
• Molecular Weight: 28.9 kDa (262aa), confirmed by MALDI-TOF(molecular weight on SDS-PAGE will appear higher)
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MWCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSSGSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE FKRELTGQRL REGDGPDGPA DDGAGAQADL EHSLLVAAEK KDEGPYRMEH FRWGSPPKDK RYGGFMTSEK SQTPLVTLFK NAIIKNAYKK GE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: POMC proopiomelanocortin [ Homo sapiens ]
• Synonyms: ACTH; adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; alpha-MSH; beta-endorphin; beta-lipotropin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; CLIP; corticotropin-like intermediary peptide; Corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; LPH; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; MSH; NPP; OTTHUMP00000200965; POC; POMC; pro-ACTH-endorphin; proopiomelanocortin; pro-opiomelanocortin; proopiomelanocortin preproprotein; proopiomelanocortin1
• Gene ID: 5443
• mRNA Refseq: NM_001035256
• Protein Refseq: NP_001030333
• MIM: 176830
• UniProt ID: P01189
• Chromosome Location: 2p23.3
• Pathway: Adipocytokine signaling pathway; Class A/1 (Rhodopsin-like receptors); G alpha (i) signalling events; G alpha (s) signalling events; G-protein activation; GPCR downstream signaling; GPCR ligand binding; Glucocorticoid receptor regulatory network; Melanogenesis; Metabolism of amino acids and derivatives; Opioid Signalling; Peptide hormone biosynthesis; Peptide ligand-binding receptors; Signaling by GPCR; Syndecan-3-mediated signaling events.
• Function: G-protein-coupled receptor binding; receptor binding; hormone activity; type 1 melanocortin receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor binding