Recombinant Human Protein Kinase D2

Species :	Human
Source :	Sf9 Cells
Tag :	GST
Description :	PRKD2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Biological Activity :	259 nmol phosphate incorporated into CREBtide substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Molecular Weight :	130.0 kDa
Form :	Recombinant proteins in storage buffer (50 mMTris-HCl, pH 7.5, 150 mMNaCl, 0.25 mM DTT, 0.1 MmEGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Appearance :	Liquid
Purity :	≥80% by SDS-PAGE
Storage :	Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoidrepeated thawing and freezing.
Pathways :	T Cell Receptor Signaling Pathway

Gene Name	PRKD2 protein kinase D2 [ Homo sapiens ]
Official Symbol	PRKD2
Synonyms	PRKD2; protein kinase D2; PKD2; HSPC187; serine/threonine-protein kinase D2; nPKC-D2; EC 2.7.11.13; DKFZP586E0820
Gene ID	25865
mRNA Refseq	NM_001079880
Protein Refseq	NP_001073349
MIM	607074
UniProt ID	Q9BZL6
Chromosome Location	19q13.3
Function	ATP binding; metal ion binding; nucleotide binding; protein kinase C activity; protein serine/threonine kinase activity; transferase activity