Recombinant Human Protein Kinase N1, GST-His

• Cat.No.: PKN1-704H
• Product Overview: Recombinant Human PKN1, Amino acids M1-C942, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells.MW = 136,910 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-942 a.a.
• Description: The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PRK1 was confirmed as PRK1 by specific Western Blotting using anti PRK1 antibody.
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.164 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 2 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PKN1 protein kinase N1 [ Homo sapiens ]
• Synonyms: PKN1; protein kinase N1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; MGC46204; PKN-ALPHA; serine/threonine-protein kinase N1; protein kinase C-like 1; protein kinase PKN-alpha; protein kinase C-like PKN; serine-threonine kinase N; protease-activated kinase 1; protein kinase C-related kinase 1; protein-kinase C-related kinase 1; serine-threonine protein kinase N; serine/threonine protein kinase N; EC 2.7.11.13
• Gene ID: 5585
• mRNA Refseq: NM_002741
• Protein Refseq: NP_002732
• MIM: 601032
• UniProt ID: Q16512
• Chromosome Location: 19p13.1-p12
• Function: ATP binding; GTP-Rho binding; Rac GTPase binding; androgen receptor binding; chromatin binding; histone binding; histone deacetylase binding; histone kinase activity (H3-T11 specific); ligand-dependent nuclear receptor transcription coactivator activity; ligand-dependent nuclear receptor transcription coactivator activity; nucleotide binding; protein binding; protein kinase C activity; protein kinase C binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity