Recombinant Human PSMB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PSMB1-1912H
• Product Overview: PSMB1 MS Standard C13 and N15-labeled recombinant protein (NP_002784) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.
• Molecular Mass: 26.5 kDa
• AA Sequence: MLSSTAMYSAAGRDLGMEPHRAAGPLQLRFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PSMB1 proteasome 20S subunit beta 1 [ Homo sapiens (human) ]
• Official Symbol: PSMB1
• Synonyms: PSMB1; proteasome (prosome, macropain) subunit, beta type, 1; proteasome subunit beta type-1; HC5; PMSB1; macropain subunit C5; proteasome gamma chain; proteasome subunit HC5; proteasome component C5; proteasome beta 1 subunit; multicatalytic endopeptidase complex subunit C5; PSC5; FLJ25321; KIAA1838
• Gene ID: 5689
• mRNA Refseq: NM_002793
• Protein Refseq: NP_002784
• MIM: 602017
• UniProt ID: P20618