Recombinant Human PTBP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PTBP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PTBP1-5835H
Product Overview : PTBP1 MS Standard C13 and N15-labeled recombinant protein (NP_002810) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 59.6 kDa
AA Sequence : MDGIVPDIAV GTKRGSDELF STCVTNGPFI MSSNSASAAN GNDSKKFKGD SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA LQAVNSVQSG NLALAASAAA VDAGMAMAGQ SPVLRIIVEN LFYPVTLDVL HQIFSKFGTV LKIITFTKNN QFQALLQYAD PVSAQHAKLS LDGQNIYNAC CTLRIDFSKL TSLNVKYNND KSRDYTRPDL PSGDSQPSLD QTMAAAFGAP GIISASPYAG AGFPPTFAIP QAAGLSVPNV HGALAPLAIP SAAAAAAAAG RIAIPGLAGA GNSVLLVSNL NPERVTPQSL FILFGVYGDV QRVKILFNKK ENALVQMADG NQAQLAMSHL NGHKLHGKPI RITLSKHQNV QLPREGQEDQ GLTKDYGNSP LHRFKKPGSK NFQNIFPPSA TLHLSNIPPS VSEEDLKVLF SSNGGVVKGF KFFQKDRKMA LIQMGSVEEA VQALIDLHNH DLGENHHLRV SFSKSTITRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PTBP1 polypyrimidine tract binding protein 1 [ Homo sapiens (human) ]
Official Symbol : PTBP1
Synonyms : PTBP1; polypyrimidine tract binding protein 1; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), PTB; polypyrimidine tract-binding protein 1; heterogeneous nuclear ribonucleoprotein I; HNRNP I; HNRPI; pPTB; PTB 1; PTB2; PTB3; PTB4; hnRNP I; RNA-binding protein; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); PTB; PTB-1; PTB-T; HNRNPI; HNRNP-I; MGC8461; MGC10830;
Gene ID : 5725
mRNA Refseq : NM_002819
Protein Refseq : NP_002810
MIM : 600693
UniProt ID : P26599

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