Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Protein Length : |
1024-1306 a.a. |
Description : |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. |
Tissue specificity : |
Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. |
Biological activity : |
114 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphateper minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM Pnpp, and 0.5 ug/ml enzyme. |
Form : |
Liquid |
Purity : |
>95% by SDS-PAGE |
Storage buffer : |
Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 75mM Sodium chloride, 25mM Tris HCl, 2mM EDTA, 10mM Glutathione, 1mM DTT, pH 8.0 |
Storage : |
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Sequence Similarities : |
Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.Contains 9 fibronectin type-III domains.Contains 1 tyrosine-protein phosphatase domain. |