Active Recombinant Full Length Human PNP Protein, His tagged

Cat.No. : PNP-2872H
Product Overview : Recombinant human PNP protein (1-289aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
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Species : Human
Source : E.coli
Tag : His
Protein Length : 1-289aa
Description : Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
Form : Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
Bio-activity : Specific activity is > 120 unit/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 μmole of inosine with inorganic phosphate per minute at pH 7.5 at 25 centigrade.
Molecular Mass : 34.2 kDa (309aa) confirmed by MALDI-TOF
AASequence : MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Enzyme Activity
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/mL (determined by Bradford assay)
Gene Name PNP purine nucleoside phosphorylase [ Homo sapiens (human) ]
Official Symbol PNP
Synonyms PNP; purine nucleoside phosphorylase; NP, nucleoside phosphorylase; PUNP; inosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase; NP; PRO1837; FLJ94043; FLJ97288; FLJ97312; MGC117396; MGC125915; MGC125916
Gene ID 4860
mRNA Refseq NM_000270
Protein Refseq NP_000261
MIM 164050
UniProt ID P00491

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