Recombinant Human QTRT1, His-tagged

• Cat.No.: QTRT1-79H
• Product Overview: Recombinant QTRT1 was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: QTRT1 also known as queuine tRNA-ribosyltransferase. QTRT1 interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate buffered saline (pH7.4) containing 30% glycerol, 1mM DTT
• Molecular Mass: 46.7kDa (428aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMAGAA TQASLESAPR IMRLVAECSR SRARAGELWL PHGTVATPVF MPVGTQATMK GITTEQLDAL GCRICLGNTY HLGLRPGPEL IQKANGLHGF MNWPHNLLTD SGGFQMVSLV SLSEVTEEGV RFRSPYDGNE TLLSPEKSVQ IQNALGSDII MQLDDVVSST VTGPRVEEAM YRSIRWLDRC IAAHQRPDKQ NLFAIIQGGL DADLRATCLE EMTKRDVPGF AIGGLSGGES KSQFWRMVAL STSRLPKDKP RYLMGVGYAT DLVVCVALGC DMFDCVFPTR TARFGSALVP TGNLQLRKKV FEKDFGPIDP ECTCPTCQKH SRAFLHALLH SDNTAALHHL TVHNIAYQLQ LMSAVRTSIV EKRFPDFVRD FMGAMYGDPT LCPTWATDAL ASVGITLG
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: QTRT1 queuine tRNA-ribosyltransferase 1 [ Homo sapiens ]
• Official Symbol: QTRT1
• Synonyms: QTRT1; queuine tRNA-ribosyltransferase 1; queuine tRNA-ribosyltransferase; TGT; tRNA guanine transglycosylase; TGT, 43-KD subunit; TGT, catalytic subunit; guanine insertion enzyme; tRNA-guanine transglycosylase; TGUT; FP3235
• Gene ID: 81890
• mRNA Refseq: NM_031209
• Protein Refseq: NP_112486
• MIM: 609615
• UniProt ID: Q9BXR0
• Chromosome Location: 19p13.3
• Function: metal ion binding; contributes_to queuine tRNA-ribosyltransferase activity; queuine tRNA-ribosyltransferase activity; transferase activity, transferring glycosyl groups