| Description : |
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
145.1 kDa |
| AA Sequence : |
MQKGIRLNDGHVASLGLLARKDGTR KGYLSKRSSDNTKWQTKWFALLQNL LFYFESDSSSRPSGLYLLEGCVCDR APSPKPALSAKEPLEKQHYFTVNFS HENQKALELRTEDAKDCDEWVAAIA HASYRTLATEHEALMQKYLHLLQIV ETEKTVAKQLRQQIEDGEIEIERLK AEITSLLKDNERIQSTQTVAPNDED SDIKKIKKVQSFLRGWLCRRKWKTI IQDYIRSPHADSMRKRNQVVFSMLE AEAEYVQQLHILVNNFLRPLRMAAS SKKPPITHDDVSSIFLNSETIMFLH QIFYQGLKARISSWPTLVLADLFDI LLPMLNIYQEFVRNHQYSLQILAHC KQNRDFDKLLKHYEAKPDCEERTLE TFLTYPMFQIPRYILTLHELLAHTP HEHVERNSLDYAKSKLEELSRIMHD EVSETENIRKNLAIERMIIEGCEIL LDTSQTFVRQGSLIQVPMSEKGKIT RGRLGSLSLKKEGERQCFLFSKHLI ICTRGSGGKLHLTKNGVISLIDCTL LEEPESTEEEAKGSGQDIDHLDFKI GVEPKDSPPFTVILVASSRQEKAAW TSDISQCVDNIRCNGLMMNAFEENS KVTVPQMIKRTREGTREAEMSRSDA SLYCDDVDIRFSKTMNSCKVLQIRY ASVERLLERLTDLRFLSIDFLNTFL HSYRVFTTAIVVLDKLITIYKKPIS AIPARWLRSLELLFASGQNNKLLYG EPPKSPRATRKFSSPPPLSITKTSS PSRRRKLSLNIPIITGGKALDLAAL SCNSNGYTSMYSAMSPFSKATLDTS KLYVSSSFTNKIPDEGDTTPEKPED PSALSKQSSEVSMREESDIDQNQSD DGDTETSPTKSPTTPKSVKNKNSSE FPLFSYNNGVVMTSCRELDNNRSAL SAASAFAIATAGANEGTPNKEKYRR MSLASAGFPPDQRNGDKEFVIRRAA TNRVLNVLRHWVSKHSQDFETNDEL KCKVIGFLEEVMHDPELLTQERKAA ANIIRTLTQEDPGDNQITLEEITQM AEGVKAEPFENHSALEIAEQLTLLD HLVFKKIPYEEFFGQGWMKLEKNER TPYIMKTTKHFNDISNLIASEIIRN EDINARVSAIEKWVAVADICRCLHN YNAVLEITSSMNRSAIFRLKKTWLK VSKQTKALIDKLQKLVSSEGRFKNL REALKNCDPPCVPYLGMYLTDLAFI EEGTPNYTEDGLVNFSKMRMISHII REIRQFQQTAYKIEHQAKVTQYLLD QSFVMDEESLYESSLRIEPKLPTTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
