| Description : |
RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
40.3 kDa |
| AA Sequence : |
MVKLFIGNLPREATEQEIRSLFEQY GKVLECDIIKNYGFVHIEDKTAAED AIRNLHHYKLHGVNINVEASKNKSK TSTKLHVGNISPTCTNKELRAKFEE YGPVIECDIVKDYAFVHMERAEDAV EAIRGLDNTEFQGKRMHVQLSTSRL RTAPGMGDQSGCYRCGKEGHWSKEC PIDRSGRVADLTEQYNEQYGAVRTP YTMSYGDSLYYNNAYGALDAYYKRC RAARSYEAVAAAAASVYNYAEQTLS QLPQVQNTAMASHLTSTSLDPYDRH LLPTSGAAATAAAAAAAAAAVTAAS TSYYGRDRSPLRRATAPVPTVGEGY GYGHESELSQASAAARNSLYDMARY EREQYADRARYSAFSGPTRTRPLEQ KLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
