Product overview : |
Recombinant Human RBP4 (P02753) aa. (Ala18~Leu201) fused with His tag was produced in HEK293F cells. |
Description : |
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. |
Source : |
HEK293F |
Species : |
Human |
Tag : |
His |
Form : |
Freeze-dried powder |
Molecular Mass : |
Predicted Molecular Mass: 22.7kDa; Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. |
Protein length : |
Ala18~Leu201 |
Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
Purity : |
> 95% |
Characteristic : |
Isoelectric point is 5.3. |
Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200ug/mL |
Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300. |
Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |