Recombinant Human RCHY1 Protein, Myc/DDK-tagged

• Cat.No.: RCHY1-234H
• Product Overview: Recombinant protein of human ring finger and CHY zinc finger domain containing 1 (RCHY1), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 29.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: RCHY1 ring finger and CHY zinc finger domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: RCHY1
• Synonyms: RCHY1; ring finger and CHY zinc finger domain containing 1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996; RING finger and CHY zinc finger domain-containing protein 1; CH-rich interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; androgen-receptor N-terminal-interacting protein; p53-induced protein with a RING-H2 domain; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; zinc finger protein 363; zinc finger, CHY-type; EC 2.3.2.27
• Gene ID: 25898
• mRNA Refseq: NM_015436
• Protein Refseq: NP_056251
• MIM: 607680
• UniProt ID: Q96PM5