Recombinant Human REN Protein, His-tagged

• Cat.No.: REN-11H
• Product Overview: Recombinant human Renin (24-406aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 43.1 kDa
• Identity: 389aa
• AA Sequence: LPTDTTTFKRIFLKRMPSIRESLKE RGVDMARLGPEWSQPMKRLTLGNTT SSVILTNYMDTQYYGEIGIGTPPQT FKVVFDTGSSNVWVPSSKCSRLYTA CVYHKLFDASDSSSYKHNGTELTLR YSTGTVSGFLSQDIITVGGITVTQM FGEVTEMPALPFMLAEFDGVVGMGF IEQAIGRVTPIFDNIISQGVLKEDV FSFYYNRDSENSQSLGGQIVLGGSD PQHYEGNFHYINLIKTGVWQIQMKG VSVGSSTLLCEDGCLALVDTGASYI SGSTSSIEKLMEALGAKKRLFDYVV KCNEGPTLPDISFHLGGKEYTLTSA DYVFQESYSSKKLCTLAIHAMDIPP PTGPTWALGATFIRKFYTEFDRRNN RIGFALAR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: REN renin [ Homo sapiens (human) ]
• Official Symbol: REN
• Synonyms: REN; renin; RTD; HNFJ2; ADTKD4; renin; angiotensin-forming enzyme; angiotensinogenase; renin precursor, renal; EC 3.4.23.15
• Gene ID: 5972
• mRNA Refseq: NM_000537
• Protein Refseq: NP_000528
• MIM: 179820
• UniProt ID: P00797