Active Recombinant Human REN Protein, His-tagged

• Cat.No.: REN-1208H
• Product Overview: Active Recombinant Human REN Protein(25-406 aa), fused with N-terminal His tag, was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 25-406 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 5% Trehalose.
• Bio-activity: Renin is an enzyme that plays a crucial role in the regulation of blood pressure and electrolyte balance by activating the renin-angiotensin-aldosterone system (RAAS). It is secreted by the kidneys in response to low blood pressure or low sodium levels. The activity of recombinant human REN is measured by its ability to cleave a fluorogenic peptide substrate Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg in the assay buffer 50 mM Sodium Acetate, 150 mM NaCl, 2 mM EDTA, pH 5.0. Recombinant human REN was diluted to 200ug/ml in the activation buffer 5 mM Tris, 15 mM NaCl, 1 mM CaCl2, 0.005% Brij-35, pH 7.5, then activated by Trypsin in a final concentration of 4ug/ml incubated at 37°C for 1 hours. Stop activation with PMSF at 1 mM and incubate at room temperature for 30 min.The activated rhREN is diluted toto different concentrations in assay buffer. Loading into a black well plate 50 µL rhREN and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhREN. Then read at excitiation and emission wavelengths of 350 nm and 490 nm, respectively, in kinetic mode for 5 minutes. The specific activity of rhREN is >13 pmol/min/µg.
• Molecular Mass: 43.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >97%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: REN renin [ Homo sapiens ]
• Official Symbol: REN
• Synonyms: REN; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; HNFJ2; FLJ10761
• Gene ID: 5972
• mRNA Refseq: NM_000537
• Protein Refseq: NP_000528
• MIM: 179820
• UniProt ID: P00797