Recombinant Human Retinol Dehydrogenase 12 (all-trans/9-cis/11-cis), His-tagged

• Cat.No.: RDH12-1371H
• Product Overview: Recombinanthuman RDH12protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RDH12 belongs to theshort-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed,mostly in eye, kidney, brain, skeletal muscle and stomach. It is anNADPH-dependent retinal reductase whose highest activity is toward 9-cis andall-trans-retinol. RDH12 also plays a role in the metabolism of short-chainaldehydes but does not exhibit steroid dehydrogenase activity.
• Form: Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 2 mM DTT, 40% glycerol, 0.2M NaCl.
• Molecular Weight: 33.5 kDa(303 aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS-PAGE
• Concentration: 0.25mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMGKVVV ITGANTGIGK ETARELASRG ARVYIACRDVLKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFLAEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKIPFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSPFVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPRARNNKTAERL WNVSCELLGI RWE
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolicpathways; Retinol metabolism; Visual signal transduction; Vitamin A andcarotenoid metabolism

Gene Information

• Gene Name: RDH12 retinol dehydrogenase 12(all-trans/9-cis/11-cis) [ Homo sapiens ]
• Official Symbol: RDH12
• Synonyms: RDH12; retinoldehydrogenase 12 (all-trans/9-cis/11-cis); LCA3; LCA13; SDR7C2;FLJ30273; retinoldehydrogenase 12; all-trans and 9-cis retinol dehydrogenase; retinoldehydrogenase 12, all-trans and 9-cis; short chain dehydrogenase/reductasefamily 7C, member 2
• Gene ID: 145226
• mRNA Refseq: NM_152443
• Protein Refseq: NP_689656
• MIM: 608830
• UniProt ID: Q96NR8
• Chromosome Location: 14q24.1
• Function: binding;oxidoreductase activity; retinol dehydrogenase activity