Recombinant Human RFC2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RFC2-1928H
• Product Overview: RFC2 MS Standard C13 and N15-labeled recombinant protein (NP_002905) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 35.2 kDa
• AA Sequence: MEVEAVCGGAGEVEAQDSDPAPAFS KAPGSAGHYELPWVEKYRPVKLNEI VGNEDTVSRLEVFAREGNVPNIIIA GPPGTGKTTSILCLARALLGPALKD AMLELNASNDSMTDGAQQALRRTME IYSKTTRFALACNASDKIIEPIQSR CAVLRYTKLTDAQILTRLMNVIEKE RVPYTDDGLEAIIFTAQGDMRQALN NLQSTFSGFGFINSENVFKVCDEPH PLLVKEMIQHCVNANIDEAYKILAH LWHLGYSPEDIIGNIFRVCKTFQMA EYLKLEFIKEIGYTHMKIAEGVNSL LQMAGLLARLCQKTMAPVASTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RFC2 replication factor C subunit 2 [ Homo sapiens (human) ]
• Official Symbol: RFC2
• Synonyms: RFC2; replication factor C (activator 1) 2, 40kDa; replication factor C (activator 1) 2 (40kD); replication factor C subunit 2; A1; activator 1; RFC40; A1 40 kDa subunit; RF-C 40 kDa subunit; activator 1 subunit 2; replication factor C 2; activator 1 40 kDa subunit; MGC3665
• Gene ID: 5982
• mRNA Refseq: NM_002914
• Protein Refseq: NP_002905
• MIM: 600404
• UniProt ID: P35250