Recombinant Human RFC3 Protein, His-tagged

• Cat.No.: RFC3-2653H
• Product Overview: Recombinant Human RFC3 Protein (Met1-Pro288) with a N-His tag was expressed in E. coli.

Specification

• Description: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.4 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Met1-Pro288
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: RFC3 replication factor C (activator 1) 3, 38kDa [ Homo sapiens (human) ]
• Official Symbol: RFC3
• Synonyms: RFC3; replication factor C (activator 1) 3, 38kDa; replication factor C (activator 1) 3 (38kD); replication factor C subunit 3; A1 38 kDa subunit; MGC5276; RFC; 38 kD subunit; RFC38; RFC, 38 kD subunit; RF-C 38 kDa subunit; activator 1 subunit 3; activator 1 38 kDa subunit; replication factor C 38 kDa subunit
• Gene ID: 5983
• mRNA Refseq: NM_002915
• Protein Refseq: NP_002906
• MIM: 600405
• UniProt ID: P40938