Recombinant Human RFC4 Protein, Myc/DDK-tagged

• Cat.No.: RFC4-293H
• Product Overview: Recombinant protein of human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 2 with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 39.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: RFC4 replication factor C subunit 4 [ Homo sapiens (human) ]
• Official Symbol: RFC4
• Synonyms: RFC4; replication factor C subunit 4; A1; RFC37; replication factor C subunit 4; A1 37 kDa subunit; RF-C 37 kDa subunit; RFC 37 kDa subunit; activator 1 37 kDa subunit; activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; replication factor C 37 kDa subunit
• Gene ID: 5984
• mRNA Refseq: NM_181573
• Protein Refseq: NP_853551
• MIM: 102577
• UniProt ID: P35249