Recombinant Human Ribonuclease P/MRP 30kDa Subunit, His-tagged

• Cat.No.: RPP30-946H
• Product Overview: Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 31.8kDa (291aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5"-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl, 1mM EDTA.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGSMAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RPP30 ribonuclease P/MRP 30kDa subunit [ Homo sapiens ]
• Synonyms: ribonuclease P/MRP 30kDa subunit; TSG15; FLJ38491; RP11-320F15.1; RPP30; ribonuclease P protein subunit p30; RNase P subunit 2; OTTHUMP00000020082; OTTHUMP00000020083; RNaseP protein p30; ribonuclease P (30kD) (RPP30); EC 3.1.26.5
• Gene ID: 10556
• mRNA Refseq: NM_001104546
• Protein Refseq: NP_001098016
• MIM: 606115
• UniProt ID: P78346
• Chromosome Location: 10q23.31
• Function: hydrolase activity; protein binding; ribonuclease P activity; ribonuclease activity