Recombinant Human RING1 And YY1 Binding Protein, His-tagged

• Cat.No.: RYBP-6846H
• Product Overview: Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-228aa
• Description: RYBP is a member of the polycomb group (PcG). This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 20% glycerol, 1mM DTT, 100mM NaCl.
• Molecular Mass: 27.4kDa (252aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RYBP RING1 and YY1 binding protein [ Homo sapiens ]
• Official Symbol: RYBP
• Synonyms: RYBP; RING1 and YY1 binding protein; RING1 and YY1-binding protein; AAP1; apoptin associating protein 1; death effector domain associated factor; DEDAF; ring1 interactor RYBP; YEAF1; YY1 and E4TF1 associated factor 1; APAP-1; DED-associated factor; apoptin-associating protein 1; YY1 and E4TF1-associated factor 1; death effector domain-associated factor
• Gene ID: 23429
• mRNA Refseq: NM_012234
• Protein Refseq: NP_036366
• MIM: 607535
• UniProt ID: Q8N488
• Chromosome Location: 3p14.2
• Pathway: E2F transcription factor network, organism-specific biosystem
• Function: DNA binding; metal ion binding; protein binding; transcription corepressor activity; zinc ion binding