| Description : |
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
29.2 kDa |
| AA Sequence : |
MWNSGFESYGSSSYGGAGGYTQSPG GFGSPAPSQAEKKSRARAQHIVPCT ISQLLSATLVDEVFRIGNVEISQVT IVGIIRHAEKAPTNIVYKIDDMTAA PMDVRQWVDTDDTSSENTVVPPETY VKVAGHLRSFQNKKSLVAFKIMPLE DMNEFTTHILEVINAHMVLSKANSQ PSAGRAPISNPGMSEAGNFGGNSFM PANGLTVAQNQVLNLIKACPRPEGL NFQDLKNQLKHMSVSSIKQAVDFLS NEGHIYSTVDDDHFKSTDAESGPTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
