Recombinant Human RPE65 Protein, DYKDDDDK-tagged

• Cat.No.: RPE65-01H
• Product Overview: Recombinant protein from the full-length sequence of homo sapiens RPE65, retinoid isomerohydrolase (RPE65) (NM_000329), with a Whitlow linker and DYKDDDDK tag was expressed in human cells.

Specification

• Description: This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
• Source: Human cells
• Species: Human
• Tag: DYKDDDDK
• Molecular Mass: 70 kDa
• Endotoxin: < 0.1 ng/μg of protein (< 1 EU/μg)
• Purity: > 90% by SDS-PAGE gel and Coomassie Blue staining
• Applications: Antigens, WB, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only!
• Storage Buffer: Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives

Gene Information

• Gene Name: RPE65 retinoid isomerohydrolase RPE65 [ Homo sapiens (human) ]
• Official Symbol: RPE65
• Synonyms: RPE65; retinoid isomerohydrolase RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; retinoid isomerohydrolase; BCO family, member 3; RBP-binding membrane protein; RPE65, retinoid isomerohydrolase; all-trans-retinyl-palmitate hydrolase; lutein isomerase; meso-zeaxanthin isomerase; retinal pigment epithelium specific protein 65; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; retinitis pigmentosa 20 (autosomal recessive); retinol isomerase; EC 3.1.1.64; EC 5.3.3.22; ATPase, H+ transporting, lysosomal interacting protein 2; ER-localized type I transmembrane adapter; ER-localized type I transmembrane adaptor; N14F; V-ATPase M8.9 subunit; embryonic liver differentiation factor 10; prorenin receptor; renin/prorenin receptor; vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9
• Gene ID: 6121
• mRNA Refseq: NM_000329
• Protein Refseq: NP_000320
• MIM: 180069
• UniProt ID: Q16518