| Description : |
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
61.4 kDa |
| AA Sequence : |
MSIQVEHPAGGYKKLFETVEELSSP LTAHVTGRIPLWLTGSLLRCGPGLF EVGSEPFYHLFDGQALLHKFDFKEG HVTYHRRFIRTDAYVRAMTEKRIVI TEFGTCAFPDPCKNIFSRFFSYFRG VEVTDNALVNVYPVGEDYYACTETN FITKINPETLETIKQVDLCNYVSVN GATAHPHIENDGTVYNIGNCFGKNF SIAYNIVKIPPLQADKEDPISKSEI VVQFPCSDRFKPSYVHSFGLTPNYI VFVETPVKINLFKFLSSWSLWGANY MDCFESNETMGVWLHIADKKRKKYL NNKYRTSPFNLFHHINTYEDNGFLI VDLCCWKGFEFVYNYLYLANLRENW EEVKKNARKAPQPEVRRYVLPLNID KADTGKNLVTLPNTTATAILCSDET IWLEPEVLFSGPRQAFEFPQINYQK YCGKPYTYAYGLGLNHFVPDRLCKL NVKTKETWVWQEPDSYPSEPIFVSH PDALEEDDGVVLSVVVSPGAGQKPA YLLILNAKDLSEVARAEVEINIPVT FHGLFKKSTRTRPLEQKLISEEDLA ANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
