Recombinant human RPS4X, His-tagged

• Cat.No.: RPS4X-2458H
• Product Overview: Recombinant human RPS4X protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS4X is ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl(pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Mass: 32kDa (286aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMARGPKK HLKRVAAPKH WMLDKLTGVF APRPSTGPHK LRECLPLIIF LRNRLKYALT GDEVKKICMQRFIKIDGKVR TDITYPAGFM DVISIDKTGE NFRLIYDTKG RFAVHRITPE EAKYKLCKVR KIFVGTKGIP HLVTHDARTI RYPDPLIKVN DTIQIDLETG KITDFIKFDT GNLCMVTGGA NLGRIGVITN RERHPGSFDV VHVKDANGNS FATRLSNIFV IGKGNKPWIS LPRGKGIRLTIAEERDKRLA AKQSSG
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RPS4X ribosomal protein S4, X-linked [ Homo sapiens ]
• Official Symbol: RPS4X
• Synonyms: RPS4X; ribosomal protein S4, X-linked; 40S ribosomal protein S4, X isoform; 40S ribosomal protein S4; X isoform; CCG2; cell cycle gene 2; DXS306; FLJ40595; ribosomal protein S4X isoform; RPS4; S4; SCAR; SCR10; single copy abundant mRNA; single-copy abundant mRNA; single copy abundant mRNA protein
• Gene ID: 6191
• mRNA Refseq: NM_001007
• Protein Refseq: NP_000998
• MIM: 312760
• UniProt ID: P62701
• Chromosome Location: Xq13.1
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem
• Function: rRNA binding; structural constituent of ribosome