Active Recombinant Human RSPO1 Protein, His-tagged, Low Endotoxin

• Cat.No.: RSPO1-5732H
• Product Overview: Active Recombinant Human RSPO1 Protein(Q2MKA7)(Arg31-Ala263) is expressed from CHO with His tag at the C-Terminus.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 31-263 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7