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Recombinant Human SELP Protein, His-tagged

Cat.No.: SELP-474H
Product Overview: Recombinant human SELP (AAN06828.1) extracellular domain (Met 1-Ala 771) was fused with a polyhistidine tag at the C-terminus.
Source: HEK293
Species: Human
Tag: His
Predicted N Terminal: Trp 42
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The secreted recombinant human SELP consists of 741 amino acids and has a predicted molecular mass of 81.3 kDa. The apparent molecular mass of the protein is approximately 120 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: SELP selectin P (granule membrane protein 140kDa, antigen CD62) [ Homo sapiens ]
Official Symbol: SELP
Synonyms: SELP; selectin P (granule membrane protein 140kDa, antigen CD62); GRMP, selectin P (granule membrane protein 140kD, antigen CD62); P-selectin; CD62; CD62P; GMP140; PADGEM; PSEL; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; GRMP; LECAM3; FLJ45155;
Gene ID: 6403
mRNA Refseq: NM_003005
Protein Refseq: NP_002996
MIM:
UniProt ID: P16109
Chromosome Location: 1q22-q25
Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem; Malaria, organism-specific biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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